Structural basis for Ca(2+)-dependent catalysis of a cutinase-like enzyme and its engineering: application to enzymatic PET depolymerization

A cutinase-like enzyme from Saccharomonospora viridis AHK190, Cut190, can depolymerize polyethylene terephthalate (PET). As high activity at approximately 70°C is required for PET depolymerization, structure-based protein engineering of Cut190 was carried out. Crystal structure information of the Cu...

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Detalles Bibliográficos
Autor principal: Oda, Masayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Biophysical Society of Japan 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326265/
https://www.ncbi.nlm.nih.gov/pubmed/34386313
http://dx.doi.org/10.2142/biophysico.bppb-v18.018