Effects of sevoflurane and adenosine receptor antagonist on the sugammadex‐induced recovery from rocuronium‐induced neuromuscular blockade in rodent phrenic nerve–hemidiaphragm tissue specimens
Sevoflurane affects on the A1 receptor in the central nervous system and potentiates the action of neuromuscular blocking agents. In the present study, we investigated whether sevoflurane (SEVO) has the ability to potentiate the neuromuscular blocking effect of rocuronium and if the specific antagon...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326504/ https://www.ncbi.nlm.nih.gov/pubmed/34337892 http://dx.doi.org/10.1002/prp2.827 |
_version_ | 1783731840351207424 |
---|---|
author | Kim, Yong Beom Choi, Jae‐Moon Park, Chungon Choi, Hey‐Ran In, Junyong Yang, Hong‐Seuk |
author_facet | Kim, Yong Beom Choi, Jae‐Moon Park, Chungon Choi, Hey‐Ran In, Junyong Yang, Hong‐Seuk |
author_sort | Kim, Yong Beom |
collection | PubMed |
description | Sevoflurane affects on the A1 receptor in the central nervous system and potentiates the action of neuromuscular blocking agents. In the present study, we investigated whether sevoflurane (SEVO) has the ability to potentiate the neuromuscular blocking effect of rocuronium and if the specific antagonist of adenosine receptor (SLV320) can reverse this effect. In this study, phrenic nerve–hemidiaphragm tissue specimens were obtained from 40 Sprague–Dawley (SD) rats. The specimens were immersed in an organ bath filled with Krebs buffer and stimulated by a train‐of‐four (TOF) pattern using indirect supramaximal stimulation at 20 s intervals. The specimens were randomly allocated to control, 2‐chloroadenosine (CADO), SEVO, or SLV320 + SEVO groups. In the CADO and SLV320 + SEVO groups, CADO and SLV320 were added to the organ bath from the start to a concentration of 10 μM and 10 nM, respectively. We then proceeded with rocuronium‐induced blockade of >95% depression of the first twitch tension of TOF (T1) and TOF ratio (TOFR). In the SEVO and SLV320 + SEVO groups, SEVO was added to the Krebs buffer solution to concentration of 400–500 μM for 10 min. Sugammadex‐induced T1 and TOFR recovery was monitored for 30 min until >95% of T1 and >0.9 of TOFR were confirmed, and the recovery pattern was compared by plotting these data. T1 recovery in the SEVO and CADO groups was significantly delayed compared with the control and SLV320 + SEVO groups (p < .05). In conclusion, sevoflurane affects on the A1 receptor at the neuromuscular junction and delays sugammadex‐induced recovery from neuromuscular blockade. |
format | Online Article Text |
id | pubmed-8326504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-83265042021-08-06 Effects of sevoflurane and adenosine receptor antagonist on the sugammadex‐induced recovery from rocuronium‐induced neuromuscular blockade in rodent phrenic nerve–hemidiaphragm tissue specimens Kim, Yong Beom Choi, Jae‐Moon Park, Chungon Choi, Hey‐Ran In, Junyong Yang, Hong‐Seuk Pharmacol Res Perspect Original Articles Sevoflurane affects on the A1 receptor in the central nervous system and potentiates the action of neuromuscular blocking agents. In the present study, we investigated whether sevoflurane (SEVO) has the ability to potentiate the neuromuscular blocking effect of rocuronium and if the specific antagonist of adenosine receptor (SLV320) can reverse this effect. In this study, phrenic nerve–hemidiaphragm tissue specimens were obtained from 40 Sprague–Dawley (SD) rats. The specimens were immersed in an organ bath filled with Krebs buffer and stimulated by a train‐of‐four (TOF) pattern using indirect supramaximal stimulation at 20 s intervals. The specimens were randomly allocated to control, 2‐chloroadenosine (CADO), SEVO, or SLV320 + SEVO groups. In the CADO and SLV320 + SEVO groups, CADO and SLV320 were added to the organ bath from the start to a concentration of 10 μM and 10 nM, respectively. We then proceeded with rocuronium‐induced blockade of >95% depression of the first twitch tension of TOF (T1) and TOF ratio (TOFR). In the SEVO and SLV320 + SEVO groups, SEVO was added to the Krebs buffer solution to concentration of 400–500 μM for 10 min. Sugammadex‐induced T1 and TOFR recovery was monitored for 30 min until >95% of T1 and >0.9 of TOFR were confirmed, and the recovery pattern was compared by plotting these data. T1 recovery in the SEVO and CADO groups was significantly delayed compared with the control and SLV320 + SEVO groups (p < .05). In conclusion, sevoflurane affects on the A1 receptor at the neuromuscular junction and delays sugammadex‐induced recovery from neuromuscular blockade. John Wiley and Sons Inc. 2021-08-01 /pmc/articles/PMC8326504/ /pubmed/34337892 http://dx.doi.org/10.1002/prp2.827 Text en © 2021 The Authors. Pharmacology Research & Perspectives published by British Pharmacological Society and American Society for Pharmacology and Experimental Therapeutics and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Kim, Yong Beom Choi, Jae‐Moon Park, Chungon Choi, Hey‐Ran In, Junyong Yang, Hong‐Seuk Effects of sevoflurane and adenosine receptor antagonist on the sugammadex‐induced recovery from rocuronium‐induced neuromuscular blockade in rodent phrenic nerve–hemidiaphragm tissue specimens |
title | Effects of sevoflurane and adenosine receptor antagonist on the sugammadex‐induced recovery from rocuronium‐induced neuromuscular blockade in rodent phrenic nerve–hemidiaphragm tissue specimens |
title_full | Effects of sevoflurane and adenosine receptor antagonist on the sugammadex‐induced recovery from rocuronium‐induced neuromuscular blockade in rodent phrenic nerve–hemidiaphragm tissue specimens |
title_fullStr | Effects of sevoflurane and adenosine receptor antagonist on the sugammadex‐induced recovery from rocuronium‐induced neuromuscular blockade in rodent phrenic nerve–hemidiaphragm tissue specimens |
title_full_unstemmed | Effects of sevoflurane and adenosine receptor antagonist on the sugammadex‐induced recovery from rocuronium‐induced neuromuscular blockade in rodent phrenic nerve–hemidiaphragm tissue specimens |
title_short | Effects of sevoflurane and adenosine receptor antagonist on the sugammadex‐induced recovery from rocuronium‐induced neuromuscular blockade in rodent phrenic nerve–hemidiaphragm tissue specimens |
title_sort | effects of sevoflurane and adenosine receptor antagonist on the sugammadex‐induced recovery from rocuronium‐induced neuromuscular blockade in rodent phrenic nerve–hemidiaphragm tissue specimens |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8326504/ https://www.ncbi.nlm.nih.gov/pubmed/34337892 http://dx.doi.org/10.1002/prp2.827 |
work_keys_str_mv | AT kimyongbeom effectsofsevofluraneandadenosinereceptorantagonistonthesugammadexinducedrecoveryfromrocuroniuminducedneuromuscularblockadeinrodentphrenicnervehemidiaphragmtissuespecimens AT choijaemoon effectsofsevofluraneandadenosinereceptorantagonistonthesugammadexinducedrecoveryfromrocuroniuminducedneuromuscularblockadeinrodentphrenicnervehemidiaphragmtissuespecimens AT parkchungon effectsofsevofluraneandadenosinereceptorantagonistonthesugammadexinducedrecoveryfromrocuroniuminducedneuromuscularblockadeinrodentphrenicnervehemidiaphragmtissuespecimens AT choiheyran effectsofsevofluraneandadenosinereceptorantagonistonthesugammadexinducedrecoveryfromrocuroniuminducedneuromuscularblockadeinrodentphrenicnervehemidiaphragmtissuespecimens AT injunyong effectsofsevofluraneandadenosinereceptorantagonistonthesugammadexinducedrecoveryfromrocuroniuminducedneuromuscularblockadeinrodentphrenicnervehemidiaphragmtissuespecimens AT yanghongseuk effectsofsevofluraneandadenosinereceptorantagonistonthesugammadexinducedrecoveryfromrocuroniuminducedneuromuscularblockadeinrodentphrenicnervehemidiaphragmtissuespecimens |