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A protocol for analyzing the protein terminome of human cancer cell line culture supernatants

Characterization of protein termini is essential for understanding how the proteome is generated through biological processes such as post-translational proteolytic events. Here, we introduce a practical protocol for terminomics to achieve simple and sensitive N- and C-terminal peptide enrichment. W...

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Detalles Bibliográficos
Autores principales: Tsumagari, Kazuya, Chang, Chih-Hsiang, Ishihama, Yasushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327658/
https://www.ncbi.nlm.nih.gov/pubmed/34377995
http://dx.doi.org/10.1016/j.xpro.2021.100682