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A protocol for analyzing the protein terminome of human cancer cell line culture supernatants
Characterization of protein termini is essential for understanding how the proteome is generated through biological processes such as post-translational proteolytic events. Here, we introduce a practical protocol for terminomics to achieve simple and sensitive N- and C-terminal peptide enrichment. W...
Autores principales: | Tsumagari, Kazuya, Chang, Chih-Hsiang, Ishihama, Yasushi |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327658/ https://www.ncbi.nlm.nih.gov/pubmed/34377995 http://dx.doi.org/10.1016/j.xpro.2021.100682 |
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