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RNase H-dependent amplification improves the accuracy of rolling circle amplification combined with loop-mediated isothermal amplification (RCA-LAMP)

The hybrid method upon combining rolling circle amplification and loop-mediated isothermal amplification (RCA-LAMP) was developed to quantify very small amount of different type of RNAs, such as miRNAs. RCA-LAMP can help detect short sequences through padlock probe (PLP) circularization and exhibit...

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Detalles Bibliográficos
Autores principales: Hasegawa, Takema, Hapsari, Diana, Iwahashi, Hitoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8327969/
https://www.ncbi.nlm.nih.gov/pubmed/34395086
http://dx.doi.org/10.7717/peerj.11851