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Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite
Chemical decontamination during processing is used in many countries to mitigate the Campylobacter load on chicken meat. Chlorine is a commonly used sanitizer in poultry processing to limit foodborne bacterial pathogens but its efficacy is limited by high bacterial loads and organic material. Acidif...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329286/ https://www.ncbi.nlm.nih.gov/pubmed/34341355 http://dx.doi.org/10.1038/s41538-021-00103-5 |
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author | Weerasooriya, Gayani McWhorter, Andrea R. Khan, Samiullah Chousalkar, Kapil K. |
author_facet | Weerasooriya, Gayani McWhorter, Andrea R. Khan, Samiullah Chousalkar, Kapil K. |
author_sort | Weerasooriya, Gayani |
collection | PubMed |
description | Chemical decontamination during processing is used in many countries to mitigate the Campylobacter load on chicken meat. Chlorine is a commonly used sanitizer in poultry processing to limit foodborne bacterial pathogens but its efficacy is limited by high bacterial loads and organic material. Acidified sodium chlorite (ASC) is a potential alternative for poultry meat sanitization but little is known about its effects on the cellular response of Campylobacter. In this study, the sensitivity of C. jejuni isolates to ASC was established. RNAseq was performed to characterize the transcriptomic response of C. jejuni following exposure to either chlorine or ASC. Following chlorine exposure, C. jejuni induced an adaptive stress response mechanism. In contrast, exposure to ASC induced higher oxidative damage and cellular death by inhibiting all vital metabolic pathways and upregulating the genes involved in DNA damage and repair. The transcriptional changes in C. jejuni in response to ASC exposure suggest its potential as an effective sanitizer for use in the chicken meat industry. |
format | Online Article Text |
id | pubmed-8329286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83292862021-08-03 Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite Weerasooriya, Gayani McWhorter, Andrea R. Khan, Samiullah Chousalkar, Kapil K. NPJ Sci Food Article Chemical decontamination during processing is used in many countries to mitigate the Campylobacter load on chicken meat. Chlorine is a commonly used sanitizer in poultry processing to limit foodborne bacterial pathogens but its efficacy is limited by high bacterial loads and organic material. Acidified sodium chlorite (ASC) is a potential alternative for poultry meat sanitization but little is known about its effects on the cellular response of Campylobacter. In this study, the sensitivity of C. jejuni isolates to ASC was established. RNAseq was performed to characterize the transcriptomic response of C. jejuni following exposure to either chlorine or ASC. Following chlorine exposure, C. jejuni induced an adaptive stress response mechanism. In contrast, exposure to ASC induced higher oxidative damage and cellular death by inhibiting all vital metabolic pathways and upregulating the genes involved in DNA damage and repair. The transcriptional changes in C. jejuni in response to ASC exposure suggest its potential as an effective sanitizer for use in the chicken meat industry. Nature Publishing Group UK 2021-08-02 /pmc/articles/PMC8329286/ /pubmed/34341355 http://dx.doi.org/10.1038/s41538-021-00103-5 Text en © Crown 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Weerasooriya, Gayani McWhorter, Andrea R. Khan, Samiullah Chousalkar, Kapil K. Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite |
title | Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite |
title_full | Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite |
title_fullStr | Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite |
title_full_unstemmed | Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite |
title_short | Transcriptomic response of Campylobacter jejuni following exposure to acidified sodium chlorite |
title_sort | transcriptomic response of campylobacter jejuni following exposure to acidified sodium chlorite |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329286/ https://www.ncbi.nlm.nih.gov/pubmed/34341355 http://dx.doi.org/10.1038/s41538-021-00103-5 |
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