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Coffea arabica bean extract inhibits glucose transport and disaccharidase activity in Caco-2 cells

The major constituents of Coffea arabica (coffee), including caffeine, chlorogenic acid and caffeic acid, exhibit antihyperglycemic properties in in vitro and in vivo models. However, whether Coffea arabica bean extract (CBE) regulates glucose uptake activity and the underlying mechanisms involved r...

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Autores principales: Ontawong, Atcharaporn, Duangjai, Acharaporn, Srimaroeng, Chutima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329997/
https://www.ncbi.nlm.nih.gov/pubmed/34405045
http://dx.doi.org/10.3892/br.2021.1449
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author Ontawong, Atcharaporn
Duangjai, Acharaporn
Srimaroeng, Chutima
author_facet Ontawong, Atcharaporn
Duangjai, Acharaporn
Srimaroeng, Chutima
author_sort Ontawong, Atcharaporn
collection PubMed
description The major constituents of Coffea arabica (coffee), including caffeine, chlorogenic acid and caffeic acid, exhibit antihyperglycemic properties in in vitro and in vivo models. However, whether Coffea arabica bean extract (CBE) regulates glucose uptake activity and the underlying mechanisms involved remain unclear. The aim of the present study was to examine the effects of CBE on glucose absorption and identify the mechanisms involved using an in vitro model. The uptake of a fluorescent glucose analog into Caco-2 colorectal adenocarcinoma cells was determined. The expression levels of sodium glucose co-transporter 1 (SGLT1) and glucose transporter 2 (GLUT2) were evaluated. In addition, glycoside hydrolase enzyme activity was investigated. It was observed that CBE inhibited disaccharidase enzyme activity. Furthermore, CBE exerted an inhibitory effect on intestinal glucose absorption by downregulating SGLT1- and GLUT2-mediated 5' AMP-activated protein kinase phosphorylation and suppressing hepatocyte nuclear factor 1α expression. These data suggest that CBE may attenuate glucose absorption and may have potentially beneficial antihyperglycemic effects in the body; however, the mechanisms underlying the effects of CBE must be elucidated through further investigation.
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spelling pubmed-83299972021-08-16 Coffea arabica bean extract inhibits glucose transport and disaccharidase activity in Caco-2 cells Ontawong, Atcharaporn Duangjai, Acharaporn Srimaroeng, Chutima Biomed Rep Articles The major constituents of Coffea arabica (coffee), including caffeine, chlorogenic acid and caffeic acid, exhibit antihyperglycemic properties in in vitro and in vivo models. However, whether Coffea arabica bean extract (CBE) regulates glucose uptake activity and the underlying mechanisms involved remain unclear. The aim of the present study was to examine the effects of CBE on glucose absorption and identify the mechanisms involved using an in vitro model. The uptake of a fluorescent glucose analog into Caco-2 colorectal adenocarcinoma cells was determined. The expression levels of sodium glucose co-transporter 1 (SGLT1) and glucose transporter 2 (GLUT2) were evaluated. In addition, glycoside hydrolase enzyme activity was investigated. It was observed that CBE inhibited disaccharidase enzyme activity. Furthermore, CBE exerted an inhibitory effect on intestinal glucose absorption by downregulating SGLT1- and GLUT2-mediated 5' AMP-activated protein kinase phosphorylation and suppressing hepatocyte nuclear factor 1α expression. These data suggest that CBE may attenuate glucose absorption and may have potentially beneficial antihyperglycemic effects in the body; however, the mechanisms underlying the effects of CBE must be elucidated through further investigation. D.A. Spandidos 2021-09 2021-07-12 /pmc/articles/PMC8329997/ /pubmed/34405045 http://dx.doi.org/10.3892/br.2021.1449 Text en Copyright: © Ontawong et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ontawong, Atcharaporn
Duangjai, Acharaporn
Srimaroeng, Chutima
Coffea arabica bean extract inhibits glucose transport and disaccharidase activity in Caco-2 cells
title Coffea arabica bean extract inhibits glucose transport and disaccharidase activity in Caco-2 cells
title_full Coffea arabica bean extract inhibits glucose transport and disaccharidase activity in Caco-2 cells
title_fullStr Coffea arabica bean extract inhibits glucose transport and disaccharidase activity in Caco-2 cells
title_full_unstemmed Coffea arabica bean extract inhibits glucose transport and disaccharidase activity in Caco-2 cells
title_short Coffea arabica bean extract inhibits glucose transport and disaccharidase activity in Caco-2 cells
title_sort coffea arabica bean extract inhibits glucose transport and disaccharidase activity in caco-2 cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8329997/
https://www.ncbi.nlm.nih.gov/pubmed/34405045
http://dx.doi.org/10.3892/br.2021.1449
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