Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1

Superoxide Dismutase 1 (SOD1) is an antioxidant enzyme that protects the cells from radical oxygen species. To study the behavior of endogenous SOD1 under a microscope, we genetically modified H1 human embryonic stem cells (hESCs) to express SOD1 fused with a SNAP-tag, a protein tag that can be cova...

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Autores principales: Huang, Pei-San, Wen, Meng-Hsuan, Xie, Xihong, Xu, An, Lee, Dung-Fang, Chen, Tai-Yen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8330834/
https://www.ncbi.nlm.nih.gov/pubmed/34118566
http://dx.doi.org/10.1016/j.scr.2021.102415
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author Huang, Pei-San
Wen, Meng-Hsuan
Xie, Xihong
Xu, An
Lee, Dung-Fang
Chen, Tai-Yen
author_facet Huang, Pei-San
Wen, Meng-Hsuan
Xie, Xihong
Xu, An
Lee, Dung-Fang
Chen, Tai-Yen
author_sort Huang, Pei-San
collection PubMed
description Superoxide Dismutase 1 (SOD1) is an antioxidant enzyme that protects the cells from radical oxygen species. To study the behavior of endogenous SOD1 under a microscope, we genetically modified H1 human embryonic stem cells (hESCs) to express SOD1 fused with a SNAP-tag, a protein tag that can be covalently labeled with a variety of synthetic probes. The engineered homozygous clone expressing SOD1-SNAP fusion proteins has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be differentiated into all three germ layers in vitro, providing a versatile platform for imaging-based studies of SOD1.
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spelling pubmed-83308342021-08-03 Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1 Huang, Pei-San Wen, Meng-Hsuan Xie, Xihong Xu, An Lee, Dung-Fang Chen, Tai-Yen Stem Cell Res Article Superoxide Dismutase 1 (SOD1) is an antioxidant enzyme that protects the cells from radical oxygen species. To study the behavior of endogenous SOD1 under a microscope, we genetically modified H1 human embryonic stem cells (hESCs) to express SOD1 fused with a SNAP-tag, a protein tag that can be covalently labeled with a variety of synthetic probes. The engineered homozygous clone expressing SOD1-SNAP fusion proteins has normal stem cell morphology and karyotype, expresses pluripotency markers, and can be differentiated into all three germ layers in vitro, providing a versatile platform for imaging-based studies of SOD1. 2021-06-06 2021-07 /pmc/articles/PMC8330834/ /pubmed/34118566 http://dx.doi.org/10.1016/j.scr.2021.102415 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Huang, Pei-San
Wen, Meng-Hsuan
Xie, Xihong
Xu, An
Lee, Dung-Fang
Chen, Tai-Yen
Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1
title Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1
title_full Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1
title_fullStr Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1
title_full_unstemmed Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1
title_short Generation of a homozygous knock-in human embryonic stem cell line expressing SNAP-tagged SOD1
title_sort generation of a homozygous knock-in human embryonic stem cell line expressing snap-tagged sod1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8330834/
https://www.ncbi.nlm.nih.gov/pubmed/34118566
http://dx.doi.org/10.1016/j.scr.2021.102415
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