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Rapid and Efficient Gene Editing for Direct Transplantation of Naive Murine Cas9(+) T Cells
Gene editing of primary T cells is a difficult task. However, it is important for research and especially for clinical T-cell transfers. CRISPR/Cas9 is the most powerful gene-editing technique. It has to be applied to cells by either retroviral transduction or electroporation of ribonucleoprotein co...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8335400/ https://www.ncbi.nlm.nih.gov/pubmed/34367143 http://dx.doi.org/10.3389/fimmu.2021.683631 |