Efficient generation of a single-copy eft-3p::TIR1::F2A:: BFP::AID*::NLS allele in the C. elegans ttTi5605 insertion site through recombination-mediated cassette exchange

The auxin-inducible degron (AID) system is a widely used system to conditionally deplete proteins. Using CRISPR/Cas9-based genome editing in C. elegans, we recently generated a set of single-copy, tissue-specific and pan-somatic TIR1-expressing strains carrying a BFP reporter inserted in single-copy...

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Detalles Bibliográficos
Autores principales: Vo, An A., Levenson, Max T., Ragle, James Matthew, Ward, Jordan D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
Materials and Reagents
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8335552/
https://www.ncbi.nlm.nih.gov/pubmed/34355140
http://dx.doi.org/10.17912/micropub.biology.000425

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8335552/
https://www.ncbi.nlm.nih.gov/pubmed/34355140
http://dx.doi.org/10.17912/micropub.biology.000425

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