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Isolation of adult mouse hippocampal neural stem cells for fluorescence loss in photobleaching assays

This protocol describes the isolation and culturing of primary neural stem cells (NSCs) from the adult mouse hippocampus, followed by the experimental approach for fluorescence loss in photobleaching assays, previously used to characterize the presence of an endoplasmic reticulum (ER) membrane diffu...

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Detalles Bibliográficos
Autores principales: bin Imtiaz, Muhammad Khadeesh, Jessberger, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339235/
https://www.ncbi.nlm.nih.gov/pubmed/34382020
http://dx.doi.org/10.1016/j.xpro.2021.100695