Cargando…

Isolation of adult mouse hippocampal neural stem cells for fluorescence loss in photobleaching assays

This protocol describes the isolation and culturing of primary neural stem cells (NSCs) from the adult mouse hippocampus, followed by the experimental approach for fluorescence loss in photobleaching assays, previously used to characterize the presence of an endoplasmic reticulum (ER) membrane diffu...

Descripción completa

Detalles Bibliográficos
Autores principales:	bin Imtiaz, Muhammad Khadeesh, Jessberger, Sebastian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8339235/ https://www.ncbi.nlm.nih.gov/pubmed/34382020 http://dx.doi.org/10.1016/j.xpro.2021.100695

Ejemplares similares

Human neural progenitors establish a diffusion barrier in the endoplasmic reticulum membrane during cell division
por: bin Imtiaz, Muhammad Khadeesh, et al.
Publicado: (2022)

Monitoring condensate dynamics in S. cerevisiae using fluorescence recovery after photobleaching
por: Sprunger, Macy L., et al.
Publicado: (2022)

Palmitoylation of BMPR1a regulates neural stem cell fate
por: Wegleiter, Thomas, et al.
Publicado: (2019)

A Discontinuous Galerkin Model for Fluorescence Loss in Photobleaching
por: Hansen, Christian V., et al.
Publicado: (2018)

A fluorescence recovery after photobleaching protocol to measure surface diffusion of DAGLα in primary cultured cortical mouse neurons
por: Yoon, Sehyoun, et al.
Publicado: (2022)

Numerical Simulation of the Photobleaching Process in Laser-Induced Fluorescence Photobleaching Anemometer
por: Chen, Yu, et al.
Publicado: (2021)

Quantitative fluorescence loss in photobleaching for analysis of protein transport and aggregation
por: Wüstner, Daniel, et al.
Publicado: (2012)

Fluorescence Recovery After Photobleaching (FRAP) of Fluorescence Tagged Proteins in Dendritic Spines of Cultured Hippocampal Neurons
por: Zheng, Chan-Ying, et al.
Publicado: (2011)

Photobleaching and Fluorescence Recovery of RPE Bisretinoids
por: Liu, Zhao, et al.
Publicado: (2015)

Do photobleached fluorescent microtubules move?: re-evaluation of fluorescence laser photobleaching both in vitro and in growing Xenopus axon
Publicado: (1993)

Protocol for single-molecule fluorescence recovery after photobleaching microscopy to analyze the dynamics and spatial locations of nuclear transmembrane proteins in live cells
por: Tingey, Mark, et al.
Publicado: (2021)

Generation of mouse hippocampal brain organoids from primary embryonic neural stem cells
por: Ciarpella, Francesca, et al.
Publicado: (2023)

A discontinuous Galerkin model for fluorescence loss in photobleaching of intracellular polyglutamine protein aggregates
por: Hansen, Christian V., et al.
Publicado: (2018)

Recording membrane potential in adult neural stem cells as readout for stem cell activation following neural circuit stimulation in mouse hippocampal slices
por: Asrican, Brent, et al.
Publicado: (2021)

Lateral Diffusion and Exocytosis of Membrane Proteins in Cultured Neurons Assessed using Fluorescence Recovery and Fluorescence-loss Photobleaching
por: Hildick, Keri L., et al.
Publicado: (2012)

Protocol for mouse adult neural stem cell isolation and culture
por: Ahmed, Ahmed K.M.A., et al.
Publicado: (2021)

Quantitative Imaging of MS2-Tagged hTR in Cajal Bodies: Photobleaching and Photoactivation
por: Smith, Michael, et al.
Publicado: (2020)

Fluorescent tagging of endogenous proteins with CRISPR/Cas9 in primary mouse neural stem cells
por: Morrow, Christopher S., et al.
Publicado: (2021)

Protocol for the isolation of mouse muscle stem cells using fluorescence-activated cell sorting
por: Elizalde, Gabriel, et al.
Publicado: (2023)

Determining vitreous viscosity using fluorescence recovery after photobleaching
por: Srikantha, Nishanthan, et al.
Publicado: (2022)

DeepFRAP: Fast fluorescence recovery after photobleaching data analysis using deep neural networks
por: Wåhlstrand Skärström, Victor, et al.
Publicado: (2021)

Enzyme-dependent fluorescence recovery of NADH after photobleaching to assess dehydrogenase activity of isolated perfused hearts
por: Moreno, Angel, et al.
Publicado: (2017)

Chemical factors that influence nucleocytoplasmic transport: a fluorescence photobleaching study
Publicado: (1986)

Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
por: Pyle, Joseph R, et al.
Publicado: (2017)

Photobleaching Kinetics and Time-Integrated Emission of Fluorescent Probes in Cellular Membranes
por: Wüstner, Daniel, et al.
Publicado: (2014)

Using Fluorescence Recovery After Photobleaching data to uncover filament dynamics
por: Dallon, J. C., et al.
Publicado: (2022)

The Utility of Fluorescence Recovery after Photobleaching (FRAP) to Study the Plasma Membrane
por: Day, Charles A., et al.
Publicado: (2023)

Recent Applications of Fluorescence Recovery after Photobleaching (FRAP) to Membrane Bio-Macromolecules
por: Rayan, Gamal, et al.
Publicado: (2010)

Assessing cellular efficacy of bromodomain inhibitors using fluorescence recovery after photobleaching
por: Philpott, Martin, et al.
Publicado: (2014)

Correction: Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
por: Pyle, Joseph R, et al.
Publicado: (2018)

Defining the Diffusion in Model Membranes Using Line Fluorescence Recovery after Photobleaching
por: Kure, Jakob L., et al.
Publicado: (2020)

The Impact of Photobleaching on Microarray Analysis
por: von der Haar, Marcel, et al.
Publicado: (2015)

Generation of mouse hippocampal neural precursor cell lines with CRISPR/Cas9-mediated gene knockouts
por: Zocher, Sara, et al.
Publicado: (2021)

Dynamic Mode Decomposition of Fluorescence Loss in Photobleaching Microscopy Data for Model-Free Analysis of Protein Transport and Aggregation in Living Cells
por: Wüstner, Daniel
Publicado: (2022)

Analysis of the treadmilling model during metaphase of mitosis using fluorescence redistribution after photobleaching
Publicado: (1986)

Digital imaging fluorescence microscopy: spatial heterogeneity of photobleaching rate constants in individual cells
Publicado: (1985)

Optical Characterization of Neurosurgical Operating Microscopes: Quantitative Fluorescence and Assessment of PpIX Photobleaching
por: Belykh, Evgenii, et al.
Publicado: (2018)

Spectrally and spatially resolved laser‐induced photobleaching of endogenous flavin fluorescence in cardiac myocytes
por: Marcek Chorvatova, Alzbeta, et al.
Publicado: (2018)

Determination of diffusion coefficients in live cells using fluorescence recovery after photobleaching with wide-field fluorescence microscopy
por: Kitamura, Akira, et al.
Publicado: (2018)

Isolation and culture of neural stem cells from adult mouse subventricular zone for genetic and pharmacological treatments with proliferation analysis
por: Radecki, Daniel Z., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_eokqib6nqltn5ak4s138fjdlf8