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A STING-based fluorescent polarization assay for monitoring activities of cyclic dinucleotide metabolizing enzymes

Cyclic dinucleoties, such as cGAMP, c-di-GMP and c-di-AMP, are fascinating second messengers with diverse roles in both prokaryotes and eukaryotes. Consequently there is a need for simple and inexpensive methods for profiling these compounds in biological media, monitoring their synthesis or degrada...

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Detalles Bibliográficos
Autores principales: Karanja, Caroline W., Yeboah, Kofi S., Ong, Wilson W. S., Sintim, Herman O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8341399/
https://www.ncbi.nlm.nih.gov/pubmed/34458783
http://dx.doi.org/10.1039/d0cb00187b