Laminin-511-derived recombinant fragment and Rho kinase inhibitor Y-27632 facilitate serial cultivation of keratinocytes differentiated from human embryonic stem cells

INTRODUCTION: Keratinocytes derived from pluripotent stem cells have a short proliferative lifespan under conventional culture conditions that are optimized for keratinocytes. Recently, a Rho kinase inhibitor, Y-27632, had been used as a standard supplement for culture medium in which the proliferat...

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Detalles Bibliográficos
Autores principales: Takagi, Ryo, Tanuma-Takahashi, Akiko, Akiyama, Saeko, Kaneko, Wakana, Miura, Chika, Yamato, Masayuki, Shimizu, Tatsuya, Umezawa, Akihiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society for Regenerative Medicine 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342860/
https://www.ncbi.nlm.nih.gov/pubmed/34409136
http://dx.doi.org/10.1016/j.reth.2021.07.004

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8342860/
https://www.ncbi.nlm.nih.gov/pubmed/34409136
http://dx.doi.org/10.1016/j.reth.2021.07.004

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