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An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept

Endothelial and epithelial barrier function is crucial for the maintenance of physiological processes. The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ). Here, we provide an experimental workflow that yields several lay...

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Detalles Bibliográficos
Autores principales: Bartosova, Maria, Ridinger, David, Marinovic, Iva, Heigwer, Jana, Zhang, Conghui, Levai, Eszter, Westhoff, Jens H., Schaefer, Franz, Terjung, Stefan, Hildenbrand, Georg, Krunic, Damir, Bestvater, Felix, Hausmann, Michael, Schmitt, Claus Peter, Zarogiannis, Sotirios G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347178/
https://www.ncbi.nlm.nih.gov/pubmed/34360944
http://dx.doi.org/10.3390/ijms22158178