Cargando…

An Experimental Workflow for Studying Barrier Integrity, Permeability, and Tight Junction Composition and Localization in a Single Endothelial Cell Monolayer: Proof of Concept

Endothelial and epithelial barrier function is crucial for the maintenance of physiological processes. The barrier paracellular permeability depends on the composition and spatial distribution of the cell-to-cell tight junctions (TJ). Here, we provide an experimental workflow that yields several lay...

Descripción completa

Detalles Bibliográficos
Autores principales:	Bartosova, Maria, Ridinger, David, Marinovic, Iva, Heigwer, Jana, Zhang, Conghui, Levai, Eszter, Westhoff, Jens H., Schaefer, Franz, Terjung, Stefan, Hildenbrand, Georg, Krunic, Damir, Bestvater, Felix, Hausmann, Michael, Schmitt, Claus Peter, Zarogiannis, Sotirios G.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8347178/ https://www.ncbi.nlm.nih.gov/pubmed/34360944 http://dx.doi.org/10.3390/ijms22158178

Ejemplares similares

Alanyl-Glutamine Restores Tight Junction Organization after Disruption by a Conventional Peritoneal Dialysis Fluid
por: Bartosova, Maria, et al.
Publicado: (2020)

Human peritoneal tight junction, transporter and channel expression in health and kidney failure, and associated solute transport
por: Levai, Eszter, et al.
Publicado: (2023)

How peritoneal dialysis transforms the peritoneum and vasculature in children with chronic kidney disease—what can we learn for future treatment?
por: Bartosova, Maria, et al.
Publicado: (2022)

Nano-Architecture of Persistent Focal DNA Damage Regions in the Minipig Epidermis Weeks after Acute γ-Irradiation
por: Scherthan, Harry, et al.
Publicado: (2023)

Encapsulating Peritoneal Sclerosis: Pathophysiology and Current Treatment Options
por: Jagirdar, Rajesh M., et al.
Publicado: (2019)

Incorporation of Low Concentrations of Gold Nanoparticles: Complex Effects on Radiation Response and Fate of Cancer Cells
por: Dobešová, Lucie, et al.
Publicado: (2022)

Understanding Cell Model Characteristics—RNA Expression Profiling in Primary and Immortalized Human Mesothelial Cells, and in Human Vein and Microvascular Endothelial Cells
por: Marinovic, Iva, et al.
Publicado: (2022)

Localization Microscopy Analyses of MRE11 Clusters in 3D-Conserved Cell Nuclei of Different Cell Lines
por: Eryilmaz, Marion, et al.
Publicado: (2018)

A tight binding and [Formula: see text] study of monolayer stanene
por: Jiang, Liming, et al.
Publicado: (2017)

CD73 Overexpression in Podocytes: A Novel Marker of Podocyte Injury in Human Kidney Disease
por: Popovic, Zoran V., et al.
Publicado: (2021)

Dose enhancement effects of gold nanoparticles specifically targeting RNA in breast cancer cells
por: Hildenbrand, Georg, et al.
Publicado: (2018)

Polarization nano-tomography of tightly focused light landscapes by self-assembled monolayers
por: Otte, Eileen, et al.
Publicado: (2019)

Challenges and Contradictions of Metal Nano-Particle Applications for Radio-Sensitivity Enhancement in Cancer Therapy
por: Pagáčová, Eva, et al.
Publicado: (2019)

Spatial Arrangements of Connexin43 in Cancer Related Cells and Re-Arrangements under Treatment Conditions: Investigations on the Nano-Scale by Super-Resolution Localization Light Microscopy
por: Pilarczyk, Götz, et al.
Publicado: (2019)

Effect of Chum Salmon Egg Lectin on Tight Junctions in Caco-2 Cell Monolayers
por: Nemoto, Ryo, et al.
Publicado: (2015)

Editorial: Mesothelial Physiology and Pathophysiology
por: Zarogiannis, Sotirios G.
Publicado: (2018)

Commentary: Imaging Biomarkers and Pathobiological Profiling in a Rat Model of Drug-Induced Interstitial Lung Disease (DIILD) Induced by Bleomycin
por: Sinis, Sotirios I., et al.
Publicado: (2021)

Challenges for Super-Resolution Localization Microscopy and Biomolecular Fluorescent Nano-Probing in Cancer Research
por: Hausmann, Michael, et al.
Publicado: (2017)

Recruitment of 53BP1 Proteins for DNA Repair and Persistence of Repair Clusters Differ for Cell Types as Detected by Single Molecule Localization Microscopy
por: Bobkova, Elizaveta, et al.
Publicado: (2018)

Angiogenic Role of Mesothelium-Derived Chemokine CXCL1 During Unfavorable Peritoneal Tissue Remodeling in Patients Receiving Peritoneal Dialysis as Renal Replacement Therapy
por: Catar, Rusan Ali, et al.
Publicado: (2022)

Utilizing 3D Printing Technology to Create Prosthetic Irises: Proof of Concept and Workflow
por: Prager, Alisa J., et al.
Publicado: (2023)

Broad-Spectrum HDAC Inhibitors Promote Autophagy through FOXO Transcription Factors in Neuroblastoma
por: Körholz, Katharina, et al.
Publicado: (2021)

A Multiparametric Assay Platform for Simultaneous In Vivo Assessment of Pronephric Morphology, Renal Function and Heart Rate in Larval Zebrafish
por: Steenbergen, Petrus J., et al.
Publicado: (2020)

Mitotic spindle: kinetochore fibers hold on tight to interpolar bundles
por: Tolić, Iva M.
Publicado: (2017)

Rapid In Vivo Validation of HDAC Inhibitor-Based Treatments in Neuroblastoma Zebrafish Xenografts
por: Wrobel, Jagoda K, et al.
Publicado: (2020)

The emerging epigenetics of PARK7 and its implication in neurodegenerative disease
por: Vavougios, George D., et al.
Publicado: (2018)

Molecular Mechanisms of Peritoneal Membrane Pathophysiology
por: Zarogiannis, Sotirios G., et al.
Publicado: (2022)

Localisation Microscopy of Breast Epithelial ErbB-2 Receptors and Gap Junctions: Trafficking after γ-Irradiation, Neuregulin-1β, and Trastuzumab Application
por: Pilarczyk, Götz, et al.
Publicado: (2017)

Conservation of k-mer Composition and Correlation Contribution between Introns and Intergenic Regions of Animalia Genomes
por: Sievers, Aaron, et al.
Publicado: (2018)

Eukaryotic Genomes Show Strong Evolutionary Conservation of k-mer Composition and Correlation Contributions between Introns and Intergenic Regions
por: Sievers, Aaron, et al.
Publicado: (2021)

Elucidation of the Clustered Nano-Architecture of Radiation-Induced DNA Damage Sites and Surrounding Chromatin in Cancer Cells: A Single Molecule Localization Microscopy Approach
por: Hausmann, Michael, et al.
Publicado: (2021)

Proof-of-Concept Workflow for Establishing Reference Intervals of Human Urine Proteome for Monitoring Physiological and Pathological Changes
por: Leng, Wenchuan, et al.
Publicado: (2017)

A proof of concept reinforcement learning based tool for non parametric population pharmacokinetics workflow optimization
por: Otalvaro, J. D., et al.
Publicado: (2022)

Therapeutic targeting of chronic kidney disease-associated DAMPs differentially contributing to vascular pathology
por: Mazzarino, Morgane, et al.
Publicado: (2023)

Carbon Nanotubes and Other Engineered Nanoparticles Induced Pathophysiology on Mesothelial Cells and Mesothelial Membranes
por: Sinis, Sotirios I., et al.
Publicado: (2018)

CD24 regulated gene expression and distribution of tight junction proteins is associated with altered barrier function in oral epithelial monolayers
por: Ye, Ping, et al.
Publicado: (2009)

Calcium Channels and Oxidative Stress Mediate a Synergistic Disruption of Tight Junctions by Ethanol and Acetaldehyde in Caco-2 Cell Monolayers
por: Samak, Geetha, et al.
Publicado: (2016)

Aeromonas sobria Serine Protease Degrades Several Protein Components of Tight Junctions and Assists Bacterial Translocation Across the T84 Monolayer
por: Ueda, Mitsunobu, et al.
Publicado: (2022)

Lactobacillus casei and Epidermal Growth Factor Prevent Osmotic Stress-Induced Tight Junction Disruption in Caco-2 Cell Monolayers
por: Samak, Geetha, et al.
Publicado: (2021)

Hesperidin enhances intestinal barrier function in Caco‐2 cell monolayers via AMPK‐mediated tight junction‐related proteins
por: Park, Ha‐Young, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_1q01ru0c6jqso71t8771ciiut7