A protocol for whole-mount immuno-coupled hybridization chain reaction (WICHCR) in zebrafish embryos and larvae

Characterizing mRNA and protein expression with temporal and spatial resolution is a valuable component of nearly every developmental study. Here, we describe a protocol that combines in situ hybridization chain reaction (HCR) and immunofluorescence, allowing for the detection of mRNAs and proteins...

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Detalles Bibliográficos
Autores principales: Ibarra-García-Padilla, Rodrigo, Howard, Aubrey Gaylon Adam, Singleton, Eileen Willey, Uribe, Rosa Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348268/
https://www.ncbi.nlm.nih.gov/pubmed/34401776
http://dx.doi.org/10.1016/j.xpro.2021.100709

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348268/
https://www.ncbi.nlm.nih.gov/pubmed/34401776
http://dx.doi.org/10.1016/j.xpro.2021.100709

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