Protocol for detecting chromatin dynamics and screening chromatin relaxer by FRAP assay

We describe a fluorescence recovery after photobleaching (FRAP) protocol for assessing the dynamics of heterochromatin/euchromatin and identifying chromatin relaxers for cell fate transition. Here, we developed a system to track heterochromatin foci with HP1α-cherry and performed FRAP assay of H1-GF...

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Detalles Bibliográficos
Autores principales: Long, Qi, Qi, Juntao, Li, Wei, Zhou, Yanshuang, Chen, Keshi, Wu, Hao, Liu, Xingguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348307/
https://www.ncbi.nlm.nih.gov/pubmed/34401775
http://dx.doi.org/10.1016/j.xpro.2021.100706

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348307/
https://www.ncbi.nlm.nih.gov/pubmed/34401775
http://dx.doi.org/10.1016/j.xpro.2021.100706

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