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Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria

Carnitine palmitoyltransferase-1 (CPT-1) is a rate-controlling enzyme for long-chain fatty acid oxidation. This manuscript provides protocols for measuring CPT-1-mediated respiration in permeabilized, adherent cell monolayers and mitochondria freshly isolated from tissue, along with examples to asse...

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Detalles Bibliográficos
Autores principales: Yang, Krista, Doan, Mary T., Stiles, Linsey, Divakaruni, Ajit S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348308/
https://www.ncbi.nlm.nih.gov/pubmed/34401773
http://dx.doi.org/10.1016/j.xpro.2021.100687