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Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria
Carnitine palmitoyltransferase-1 (CPT-1) is a rate-controlling enzyme for long-chain fatty acid oxidation. This manuscript provides protocols for measuring CPT-1-mediated respiration in permeabilized, adherent cell monolayers and mitochondria freshly isolated from tissue, along with examples to asse...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8348308/ https://www.ncbi.nlm.nih.gov/pubmed/34401773 http://dx.doi.org/10.1016/j.xpro.2021.100687 |