A yeast FRET biosensor enlightens cAMP signaling

The cAMP-PKA signaling cascade in budding yeast regulates adaptation to changing environments. We developed yEPAC, a FRET-based biosensor for cAMP measurements in yeast. We used this sensor with flow cytometry for high-throughput single cell-level quantification during dynamic changes in response to...

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Detalles Bibliográficos
Autores principales: Botman, Dennis, O’Toole, Tom G., Goedhart, Joachim, Bruggeman, Frank J., van Heerden, Johan H., Teusink, Bas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2021
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351543/
https://www.ncbi.nlm.nih.gov/pubmed/33881352
http://dx.doi.org/10.1091/mbc.E20-05-0319

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8351543/
https://www.ncbi.nlm.nih.gov/pubmed/33881352
http://dx.doi.org/10.1091/mbc.E20-05-0319

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