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Purification of active human vacuolar H(+)-ATPase in native lipid-containing nanodiscs

Vacuolar H(+)-ATPases (V-ATPases) are large, multisubunit proton pumps that acidify the lumen of organelles in virtually every eukaryotic cell and in specialized acid-secreting animal cells, the enzyme pumps protons into the extracellular space. In higher organisms, most of the subunits are expresse...

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Detalles Bibliográficos
Autores principales: Oot, Rebecca A., Yao, Yeqi, Manolson, Morris F., Wilkens, Stephan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8353480/
https://www.ncbi.nlm.nih.gov/pubmed/34270960
http://dx.doi.org/10.1016/j.jbc.2021.100964