Cargando…
Digital Image Analysis of the Proliferation Markers Ki67 and Phosphohistone H3 in Gastroenteropancreatic Neuroendocrine Neoplasms: Accuracy of Grading Compared With Routine Manual Hot Spot Evaluation of the Ki67 Index
Gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) are rare epithelial neoplasms. Grading is based on mitotic activity or the percentage of Ki67-positive cells in a hot spot. Routine methods have poor intraobserver and interobserver consistency, and objective measurements are lacking. This s...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354564/ https://www.ncbi.nlm.nih.gov/pubmed/33758143 http://dx.doi.org/10.1097/PAI.0000000000000934 |
_version_ | 1783736620353060864 |
---|---|
author | Lea, Dordi Gudlaugsson, Einar G. Skaland, Ivar Lillesand, Melinda Søreide, Kjetil Søreide, Jon A. |
author_facet | Lea, Dordi Gudlaugsson, Einar G. Skaland, Ivar Lillesand, Melinda Søreide, Kjetil Søreide, Jon A. |
author_sort | Lea, Dordi |
collection | PubMed |
description | Gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) are rare epithelial neoplasms. Grading is based on mitotic activity or the percentage of Ki67-positive cells in a hot spot. Routine methods have poor intraobserver and interobserver consistency, and objective measurements are lacking. This study aimed to evaluate digital image analysis (DIA) as an objective assessment of proliferation markers in GEP-NENs. A consecutive cohort of patients with automated DIA measurement of Ki67 (DIA Ki67) and phosphohistone H3 (DIA PHH3) on immunohistochemical slides was analyzed using Visiopharm image analysis software (Hoersholm, Denmark). The results were compared with the Ki67 index from routine pathology reports (pathology Ki67). The study included 159 patients (57% males). The median pathology Ki67 was 2.0% and DIA Ki67 was 4.1%. The interclass correlation coefficient of the DIA Ki67 compared with the pathology Ki67 showed an excellent agreement of 0.96 [95% confidence interval (CI): 0.94-0.96]. The observed kappa value was 0.86 (95% CI: 0.81-0.91) when comparing grades based on the same methods. PHH3 was measured in 145 (91.2%) cases. The observed kappa value was 0.74. (95% CI: 0.65-0.83) when comparing grade based on the DIA PHH3 and the pathology Ki67. The DIA Ki67 shows excellent agreement with the pathology Ki67. The DIA PHH3 measurements were more varied and cannot replace other methods for grading GEP-NENs. |
format | Online Article Text |
id | pubmed-8354564 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-83545642021-08-18 Digital Image Analysis of the Proliferation Markers Ki67 and Phosphohistone H3 in Gastroenteropancreatic Neuroendocrine Neoplasms: Accuracy of Grading Compared With Routine Manual Hot Spot Evaluation of the Ki67 Index Lea, Dordi Gudlaugsson, Einar G. Skaland, Ivar Lillesand, Melinda Søreide, Kjetil Søreide, Jon A. Appl Immunohistochem Mol Morphol Research Articles Gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) are rare epithelial neoplasms. Grading is based on mitotic activity or the percentage of Ki67-positive cells in a hot spot. Routine methods have poor intraobserver and interobserver consistency, and objective measurements are lacking. This study aimed to evaluate digital image analysis (DIA) as an objective assessment of proliferation markers in GEP-NENs. A consecutive cohort of patients with automated DIA measurement of Ki67 (DIA Ki67) and phosphohistone H3 (DIA PHH3) on immunohistochemical slides was analyzed using Visiopharm image analysis software (Hoersholm, Denmark). The results were compared with the Ki67 index from routine pathology reports (pathology Ki67). The study included 159 patients (57% males). The median pathology Ki67 was 2.0% and DIA Ki67 was 4.1%. The interclass correlation coefficient of the DIA Ki67 compared with the pathology Ki67 showed an excellent agreement of 0.96 [95% confidence interval (CI): 0.94-0.96]. The observed kappa value was 0.86 (95% CI: 0.81-0.91) when comparing grades based on the same methods. PHH3 was measured in 145 (91.2%) cases. The observed kappa value was 0.74. (95% CI: 0.65-0.83) when comparing grade based on the DIA PHH3 and the pathology Ki67. The DIA Ki67 shows excellent agreement with the pathology Ki67. The DIA PHH3 measurements were more varied and cannot replace other methods for grading GEP-NENs. Lippincott Williams & Wilkins 2021-08 2021-03-22 /pmc/articles/PMC8354564/ /pubmed/33758143 http://dx.doi.org/10.1097/PAI.0000000000000934 Text en Copyright © 2021 The Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Research Articles Lea, Dordi Gudlaugsson, Einar G. Skaland, Ivar Lillesand, Melinda Søreide, Kjetil Søreide, Jon A. Digital Image Analysis of the Proliferation Markers Ki67 and Phosphohistone H3 in Gastroenteropancreatic Neuroendocrine Neoplasms: Accuracy of Grading Compared With Routine Manual Hot Spot Evaluation of the Ki67 Index |
title | Digital Image Analysis of the Proliferation Markers Ki67 and Phosphohistone H3 in Gastroenteropancreatic Neuroendocrine Neoplasms: Accuracy of Grading Compared With Routine Manual Hot Spot Evaluation of the Ki67 Index |
title_full | Digital Image Analysis of the Proliferation Markers Ki67 and Phosphohistone H3 in Gastroenteropancreatic Neuroendocrine Neoplasms: Accuracy of Grading Compared With Routine Manual Hot Spot Evaluation of the Ki67 Index |
title_fullStr | Digital Image Analysis of the Proliferation Markers Ki67 and Phosphohistone H3 in Gastroenteropancreatic Neuroendocrine Neoplasms: Accuracy of Grading Compared With Routine Manual Hot Spot Evaluation of the Ki67 Index |
title_full_unstemmed | Digital Image Analysis of the Proliferation Markers Ki67 and Phosphohistone H3 in Gastroenteropancreatic Neuroendocrine Neoplasms: Accuracy of Grading Compared With Routine Manual Hot Spot Evaluation of the Ki67 Index |
title_short | Digital Image Analysis of the Proliferation Markers Ki67 and Phosphohistone H3 in Gastroenteropancreatic Neuroendocrine Neoplasms: Accuracy of Grading Compared With Routine Manual Hot Spot Evaluation of the Ki67 Index |
title_sort | digital image analysis of the proliferation markers ki67 and phosphohistone h3 in gastroenteropancreatic neuroendocrine neoplasms: accuracy of grading compared with routine manual hot spot evaluation of the ki67 index |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8354564/ https://www.ncbi.nlm.nih.gov/pubmed/33758143 http://dx.doi.org/10.1097/PAI.0000000000000934 |
work_keys_str_mv | AT leadordi digitalimageanalysisoftheproliferationmarkerski67andphosphohistoneh3ingastroenteropancreaticneuroendocrineneoplasmsaccuracyofgradingcomparedwithroutinemanualhotspotevaluationoftheki67index AT gudlaugssoneinarg digitalimageanalysisoftheproliferationmarkerski67andphosphohistoneh3ingastroenteropancreaticneuroendocrineneoplasmsaccuracyofgradingcomparedwithroutinemanualhotspotevaluationoftheki67index AT skalandivar digitalimageanalysisoftheproliferationmarkerski67andphosphohistoneh3ingastroenteropancreaticneuroendocrineneoplasmsaccuracyofgradingcomparedwithroutinemanualhotspotevaluationoftheki67index AT lillesandmelinda digitalimageanalysisoftheproliferationmarkerski67andphosphohistoneh3ingastroenteropancreaticneuroendocrineneoplasmsaccuracyofgradingcomparedwithroutinemanualhotspotevaluationoftheki67index AT søreidekjetil digitalimageanalysisoftheproliferationmarkerski67andphosphohistoneh3ingastroenteropancreaticneuroendocrineneoplasmsaccuracyofgradingcomparedwithroutinemanualhotspotevaluationoftheki67index AT søreidejona digitalimageanalysisoftheproliferationmarkerski67andphosphohistoneh3ingastroenteropancreaticneuroendocrineneoplasmsaccuracyofgradingcomparedwithroutinemanualhotspotevaluationoftheki67index |