Enhanced genome editing efficiency of CRISPR PLUS: Cas9 chimeric fusion proteins

Efforts to improve CRISPR-Cas9 genome editing systems for lower off-target effects are mostly at the cost of its robust on-target efficiency. To enhance both accuracy and efficiency, we created chimeric SpyCas9 proteins fused with the 5′-to-3′ exonuclease Recombination J (RecJ) or with GFP and demon...

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Detalles Bibliográficos
Autores principales: Park, Jongjin, Yoon, Jiyoung, Kwon, Daekee, Han, Mi-Jung, Choi, Sunmee, Park, Slki, Lee, Junghyuk, Lee, Kiwook, Lee, Jaehwan, Lee, Seunghee, Kang, Kyung-Sun, Choe, Sunghwa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355345/
https://www.ncbi.nlm.nih.gov/pubmed/34376729
http://dx.doi.org/10.1038/s41598-021-95406-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8355345/
https://www.ncbi.nlm.nih.gov/pubmed/34376729
http://dx.doi.org/10.1038/s41598-021-95406-8

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