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Curdlan intake changes gut microbial composition, short-chain fatty acid production, and bile acid transformation in mice

Indigestible polysaccharides, such as dietary fibers, benefit the host by improving the intestinal environment. Short-chain fatty acids (SCFAs) produced by gut microbial fermentation from dietary fibers exert various physiological effects. The bacterial polysaccharide curdlan benefits the host intes...

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Autores principales: Watanabe, Keita, Yamano, Mayu, Masujima, Yuki, Ohue-Kitano, Ryuji, Kimura, Ikuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358642/
https://www.ncbi.nlm.nih.gov/pubmed/34401531
http://dx.doi.org/10.1016/j.bbrep.2021.101095
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author Watanabe, Keita
Yamano, Mayu
Masujima, Yuki
Ohue-Kitano, Ryuji
Kimura, Ikuo
author_facet Watanabe, Keita
Yamano, Mayu
Masujima, Yuki
Ohue-Kitano, Ryuji
Kimura, Ikuo
author_sort Watanabe, Keita
collection PubMed
description Indigestible polysaccharides, such as dietary fibers, benefit the host by improving the intestinal environment. Short-chain fatty acids (SCFAs) produced by gut microbial fermentation from dietary fibers exert various physiological effects. The bacterial polysaccharide curdlan benefits the host intestinal environment, although its effect on energy metabolism and SCFA production remains unclear. Hence, this study aimed to elucidate the effect of curdlan intake on gut microbial profiles, SCFA production, and energy metabolism in a high-fat diet (HFD)-induced obese mouse model. Gut microbial composition of fecal samples from curdlan-supplemented HFD-fed mice indicated an elevated abundance of Bacteroidetes, whereas a reduced abundance of Firmicutes was noted at the phylum level compared with that in cellulose-supplemented HFD-fed mice. Moreover, curdlan supplementation resulted in an abundance of the family Bacteroidales S24-7 and Erysipelotrichaceae, and a reduction in Deferribacteres in the feces. Furthermore, curdlan supplementation elevated fecal SCFA levels, particularly butyrate. Although body weight and fat mass were not affected by curdlan supplementation in HFD-induced obese mice, HFD-induced hyperglycemia was significantly suppressed with an increase in plasma insulin and incretin GLP-1 levels. Curdlan supplementation elevated fecal bile acid and SCFA production, improved host metabolic functions by altering the gut microbial composition in mice.
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spelling pubmed-83586422021-08-15 Curdlan intake changes gut microbial composition, short-chain fatty acid production, and bile acid transformation in mice Watanabe, Keita Yamano, Mayu Masujima, Yuki Ohue-Kitano, Ryuji Kimura, Ikuo Biochem Biophys Rep Research Article Indigestible polysaccharides, such as dietary fibers, benefit the host by improving the intestinal environment. Short-chain fatty acids (SCFAs) produced by gut microbial fermentation from dietary fibers exert various physiological effects. The bacterial polysaccharide curdlan benefits the host intestinal environment, although its effect on energy metabolism and SCFA production remains unclear. Hence, this study aimed to elucidate the effect of curdlan intake on gut microbial profiles, SCFA production, and energy metabolism in a high-fat diet (HFD)-induced obese mouse model. Gut microbial composition of fecal samples from curdlan-supplemented HFD-fed mice indicated an elevated abundance of Bacteroidetes, whereas a reduced abundance of Firmicutes was noted at the phylum level compared with that in cellulose-supplemented HFD-fed mice. Moreover, curdlan supplementation resulted in an abundance of the family Bacteroidales S24-7 and Erysipelotrichaceae, and a reduction in Deferribacteres in the feces. Furthermore, curdlan supplementation elevated fecal SCFA levels, particularly butyrate. Although body weight and fat mass were not affected by curdlan supplementation in HFD-induced obese mice, HFD-induced hyperglycemia was significantly suppressed with an increase in plasma insulin and incretin GLP-1 levels. Curdlan supplementation elevated fecal bile acid and SCFA production, improved host metabolic functions by altering the gut microbial composition in mice. Elsevier 2021-08-07 /pmc/articles/PMC8358642/ /pubmed/34401531 http://dx.doi.org/10.1016/j.bbrep.2021.101095 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Watanabe, Keita
Yamano, Mayu
Masujima, Yuki
Ohue-Kitano, Ryuji
Kimura, Ikuo
Curdlan intake changes gut microbial composition, short-chain fatty acid production, and bile acid transformation in mice
title Curdlan intake changes gut microbial composition, short-chain fatty acid production, and bile acid transformation in mice
title_full Curdlan intake changes gut microbial composition, short-chain fatty acid production, and bile acid transformation in mice
title_fullStr Curdlan intake changes gut microbial composition, short-chain fatty acid production, and bile acid transformation in mice
title_full_unstemmed Curdlan intake changes gut microbial composition, short-chain fatty acid production, and bile acid transformation in mice
title_short Curdlan intake changes gut microbial composition, short-chain fatty acid production, and bile acid transformation in mice
title_sort curdlan intake changes gut microbial composition, short-chain fatty acid production, and bile acid transformation in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8358642/
https://www.ncbi.nlm.nih.gov/pubmed/34401531
http://dx.doi.org/10.1016/j.bbrep.2021.101095
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