CRISPR/Cas9 mediated T7 RNA polymerase gene knock-in in E. coli BW25113 makes T7 expression system work efficiently

T7 Expression System is a common method of ensuring tight control and high-level induced expression. However, this system can only work in some bacterial strains in which the T7 RNA Polymerase gene resides in the chromosome. In this study, we successfully introduced a chromosomal copy of the T7 RNA...

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Detalles Bibliográficos
Autores principales: Ye, Changchuan, Chen, Xi, Yang, Mengjie, Zeng, Xiangfang, Qiao, Shiyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359068/
https://www.ncbi.nlm.nih.gov/pubmed/34384456
http://dx.doi.org/10.1186/s13036-021-00270-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8359068/
https://www.ncbi.nlm.nih.gov/pubmed/34384456
http://dx.doi.org/10.1186/s13036-021-00270-9

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