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Exploring Structure and Function of Redox Intermediates in [NiFe]‐Hydrogenases by an Advanced Experimental Approach for Solvated, Lyophilized and Crystallized Metalloenzymes

To study metalloenzymes in detail, we developed a new experimental setup allowing the controlled preparation of catalytic intermediates for characterization by various spectroscopic techniques. The in situ monitoring of redox transitions by infrared spectroscopy in enzyme lyophilizate, crystals, and...

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Detalles Bibliográficos
Autores principales: Lorent, Christian, Pelmenschikov, Vladimir, Frielingsdorf, Stefan, Schoknecht, Janna, Caserta, Giorgio, Yoda, Yoshitaka, Wang, Hongxin, Tamasaku, Kenji, Lenz, Oliver, Cramer, Stephen P., Horch, Marius, Lauterbach, Lars, Zebger, Ingo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360142/
https://www.ncbi.nlm.nih.gov/pubmed/33783938
http://dx.doi.org/10.1002/anie.202100451