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Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I
The repetitive sequences of DNA centromeric regions form the structural basis for kinetochore assembly. Recently they were found to be transcriptionally active in mitosis, with their RNAs providing noncoding functions. Here we explore the role, in mouse oocytes, of transcripts generated from within...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360762/ https://www.ncbi.nlm.nih.gov/pubmed/34379093 http://dx.doi.org/10.1083/jcb.202011153 |
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author | Wu, Tianyu Lane, Simon I.R. Morgan, Stephanie L. Tang, Feng Jones, Keith T. |
author_facet | Wu, Tianyu Lane, Simon I.R. Morgan, Stephanie L. Tang, Feng Jones, Keith T. |
author_sort | Wu, Tianyu |
collection | PubMed |
description | The repetitive sequences of DNA centromeric regions form the structural basis for kinetochore assembly. Recently they were found to be transcriptionally active in mitosis, with their RNAs providing noncoding functions. Here we explore the role, in mouse oocytes, of transcripts generated from within the minor satellite repeats. Depletion of minor satellite transcripts delayed progression through meiosis I by activation of the spindle assembly checkpoint. Arrested oocytes had poorly congressed chromosomes, and centromeres were frequently split by microtubules. Thus, we have demonstrated that the centromeric RNA plays a specific role in female meiosis I compared with mitosis and is required for maintaining the structural integrity of centromeres. This may contribute to the high aneuploidy rates observed in female meiosis. |
format | Online Article Text |
id | pubmed-8360762 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-83607622022-04-04 Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I Wu, Tianyu Lane, Simon I.R. Morgan, Stephanie L. Tang, Feng Jones, Keith T. J Cell Biol Report The repetitive sequences of DNA centromeric regions form the structural basis for kinetochore assembly. Recently they were found to be transcriptionally active in mitosis, with their RNAs providing noncoding functions. Here we explore the role, in mouse oocytes, of transcripts generated from within the minor satellite repeats. Depletion of minor satellite transcripts delayed progression through meiosis I by activation of the spindle assembly checkpoint. Arrested oocytes had poorly congressed chromosomes, and centromeres were frequently split by microtubules. Thus, we have demonstrated that the centromeric RNA plays a specific role in female meiosis I compared with mitosis and is required for maintaining the structural integrity of centromeres. This may contribute to the high aneuploidy rates observed in female meiosis. Rockefeller University Press 2021-08-11 /pmc/articles/PMC8360762/ /pubmed/34379093 http://dx.doi.org/10.1083/jcb.202011153 Text en © 2021 Wu et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Report Wu, Tianyu Lane, Simon I.R. Morgan, Stephanie L. Tang, Feng Jones, Keith T. Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I |
title | Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I |
title_full | Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I |
title_fullStr | Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I |
title_full_unstemmed | Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I |
title_short | Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I |
title_sort | loss of centromeric rna activates the spindle assembly checkpoint in mammalian female meiosis i |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8360762/ https://www.ncbi.nlm.nih.gov/pubmed/34379093 http://dx.doi.org/10.1083/jcb.202011153 |
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