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Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicity
Gene expression toxicity is an important biological phenomenon and a major bottleneck in biotechnology. Escherichia coli BL21(DE3) is the most popular choice for recombinant protein production, and various derivatives have been evolved or engineered to facilitate improved yield and tolerance to toxi...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361080/ https://www.ncbi.nlm.nih.gov/pubmed/34385596 http://dx.doi.org/10.1038/s42003-021-02493-4 |