Tailoring the evolution of BL21(DE3) uncovers a key role for RNA stability in gene expression toxicity

Gene expression toxicity is an important biological phenomenon and a major bottleneck in biotechnology. Escherichia coli BL21(DE3) is the most popular choice for recombinant protein production, and various derivatives have been evolved or engineered to facilitate improved yield and tolerance to toxi...

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Detalles Bibliográficos
Autores principales: Heyde, Sophia A. H., Nørholm, Morten H. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361080/
https://www.ncbi.nlm.nih.gov/pubmed/34385596
http://dx.doi.org/10.1038/s42003-021-02493-4