A luminescence-based protocol for assessing fructose metabolism via quantification of ketohexokinase enzymatic activity in mouse or human hepatocytes

Ketohexokinase (KHK) catalyzes the first step of fructose metabolism. Inhibitors of KHK enzymatic activity are being evaluated in clinical trials for the treatment of non-alcoholic fatty liver disease (NAFLD) and diabetes. Here, we present a luminescence-based protocol to quantify KHK activity. The...

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Detalles Bibliográficos
Autores principales: Park, Se-Hyung, Helsley, Robert N., Noetzli, Leila, Tu, Ho-Chou, Wallenius, Kristina, O’Mahony, Gavin, Boucher, Jeremie, Liu, Jianming, Softic, Samir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361265/
https://www.ncbi.nlm.nih.gov/pubmed/34409309
http://dx.doi.org/10.1016/j.xpro.2021.100731

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361265/
https://www.ncbi.nlm.nih.gov/pubmed/34409309
http://dx.doi.org/10.1016/j.xpro.2021.100731

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