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Protocol for in vivo and ex vivo assessments of glucose-stimulated insulin secretion in mouse islet β cells

Pancreatic islet β cells secrete insulin in a biphasic manner when sensing high blood glucose level. This protocol describes the evaluation of different phases of insulin secretion, as well as basal, glucose-stimulated and total insulin secretion abilities, thereby enabling precise assessment of β c...

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Detalles Bibliográficos
Autores principales: Zhu, Yun-Xia, Zhou, Yun-Cai, Zhang, Yan, Sun, Peng, Chang, Xiao-Ai, Han, Xiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361272/
https://www.ncbi.nlm.nih.gov/pubmed/34409308
http://dx.doi.org/10.1016/j.xpro.2021.100728