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A sterol-defined system for quantitative studies of sterol metabolism in C. elegans
This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361321/ https://www.ncbi.nlm.nih.gov/pubmed/34409305 http://dx.doi.org/10.1016/j.xpro.2021.100710 |