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A sterol-defined system for quantitative studies of sterol metabolism in C. elegans

This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential...

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Detalles Bibliográficos
Autores principales: Trabelcy, Benjamin, Gerchman, Yoram, Sapir, Amir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361321/
https://www.ncbi.nlm.nih.gov/pubmed/34409305
http://dx.doi.org/10.1016/j.xpro.2021.100710