A sterol-defined system for quantitative studies of sterol metabolism in C. elegans

This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential...

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Detalles Bibliográficos
Autores principales: Trabelcy, Benjamin, Gerchman, Yoram, Sapir, Amir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361321/
https://www.ncbi.nlm.nih.gov/pubmed/34409305
http://dx.doi.org/10.1016/j.xpro.2021.100710
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author Trabelcy, Benjamin
Gerchman, Yoram
Sapir, Amir
author_facet Trabelcy, Benjamin
Gerchman, Yoram
Sapir, Amir
author_sort Trabelcy, Benjamin
collection PubMed
description This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. C. elegans cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, C. elegans is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol. For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020).
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spelling pubmed-83613212021-08-17 A sterol-defined system for quantitative studies of sterol metabolism in C. elegans Trabelcy, Benjamin Gerchman, Yoram Sapir, Amir STAR Protoc Protocol This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. C. elegans cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, C. elegans is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol. For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020). Elsevier 2021-08-10 /pmc/articles/PMC8361321/ /pubmed/34409305 http://dx.doi.org/10.1016/j.xpro.2021.100710 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Trabelcy, Benjamin
Gerchman, Yoram
Sapir, Amir
A sterol-defined system for quantitative studies of sterol metabolism in C. elegans
title A sterol-defined system for quantitative studies of sterol metabolism in C. elegans
title_full A sterol-defined system for quantitative studies of sterol metabolism in C. elegans
title_fullStr A sterol-defined system for quantitative studies of sterol metabolism in C. elegans
title_full_unstemmed A sterol-defined system for quantitative studies of sterol metabolism in C. elegans
title_short A sterol-defined system for quantitative studies of sterol metabolism in C. elegans
title_sort sterol-defined system for quantitative studies of sterol metabolism in c. elegans
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361321/
https://www.ncbi.nlm.nih.gov/pubmed/34409305
http://dx.doi.org/10.1016/j.xpro.2021.100710
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