Depletion of nuclear LINE1 RNA in mouse ESCs and embryos

LINE1 is the most active and abundant family of retrotransposons; it is implicated in a number of pathologies, as well as in early embryo development. We present a protocol to specifically knockdown LINE1 in mouse embryonic stem cells and embryos, including details for the nucleofection and zygote m...

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Detalles Bibliográficos
Autores principales: Percharde, Michelle, Lin, Chih-Jen, Ramalho-Santos, Miguel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361322/
https://www.ncbi.nlm.nih.gov/pubmed/34409306
http://dx.doi.org/10.1016/j.xpro.2021.100726

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8361322/
https://www.ncbi.nlm.nih.gov/pubmed/34409306
http://dx.doi.org/10.1016/j.xpro.2021.100726

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