Highly Selective, CRISPR/Cas9‐Mediated Isolation of Genes and Genomic Loci from Complex Genomes by TAR Cloning in Yeast

Here we describe an updated TAR cloning protocol for the selective and efficient isolation of any genomic fragment or gene of interest up to 280 kb in size from genomic DNA. The method exploits the special recombination machinery of the yeast Saccharomyces cerevisiae. TAR cloning is based on the hig...

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Detalles Bibliográficos
Autores principales: Kouprina, Natalay, Kim, Jung‐Hyun, Larionov, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363120/
https://www.ncbi.nlm.nih.gov/pubmed/34370406
http://dx.doi.org/10.1002/cpz1.207

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363120/
https://www.ncbi.nlm.nih.gov/pubmed/34370406
http://dx.doi.org/10.1002/cpz1.207

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