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The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus
The sperm head-to-tail coupling apparatus (HTCA) ensures sperm head-tail integrity while defective HTCA causes acephalic spermatozoa, rendering males infertile. Here, we show that CENTLEIN is indispensable for HTCA integrity and function, and that inactivation of CENTLEIN in mice leads to sperm deca...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363609/ https://www.ncbi.nlm.nih.gov/pubmed/34389728 http://dx.doi.org/10.1038/s41467-021-25227-w |
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author | Zhang, Ying Liu, Chao Wu, Bingbing Li, Liansheng Li, Wei Yuan, Li |
author_facet | Zhang, Ying Liu, Chao Wu, Bingbing Li, Liansheng Li, Wei Yuan, Li |
author_sort | Zhang, Ying |
collection | PubMed |
description | The sperm head-to-tail coupling apparatus (HTCA) ensures sperm head-tail integrity while defective HTCA causes acephalic spermatozoa, rendering males infertile. Here, we show that CENTLEIN is indispensable for HTCA integrity and function, and that inactivation of CENTLEIN in mice leads to sperm decapitation and male sterility. We demonstrate that CENTLEIN directly interacts with both SUN5 and PMFBP1, two proteins localized in the HTCA and related with acephalic spermatozoa syndrome. We find that the absence of Centlein sets SUN5 and PMFBP1 apart, the former close to the sperm head and the latter in the decapitated tail. We show that lack of Sun5 results in CENTLEIN and PMFBP1 left in the decapitated tail, while disruption of Pmfbp1 results in SUN5 and CENTLEIN left on the detached sperm head. These results demonstrate that CENTLEIN cooperating with SUN5 and PMFBP1 participates in the HTCA assembly and integration of sperm head to the tail, indicating that impairments of CENTLEIN might be associated with acephalic spermatozoa syndrome in humans. |
format | Online Article Text |
id | pubmed-8363609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-83636092021-08-19 The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus Zhang, Ying Liu, Chao Wu, Bingbing Li, Liansheng Li, Wei Yuan, Li Nat Commun Article The sperm head-to-tail coupling apparatus (HTCA) ensures sperm head-tail integrity while defective HTCA causes acephalic spermatozoa, rendering males infertile. Here, we show that CENTLEIN is indispensable for HTCA integrity and function, and that inactivation of CENTLEIN in mice leads to sperm decapitation and male sterility. We demonstrate that CENTLEIN directly interacts with both SUN5 and PMFBP1, two proteins localized in the HTCA and related with acephalic spermatozoa syndrome. We find that the absence of Centlein sets SUN5 and PMFBP1 apart, the former close to the sperm head and the latter in the decapitated tail. We show that lack of Sun5 results in CENTLEIN and PMFBP1 left in the decapitated tail, while disruption of Pmfbp1 results in SUN5 and CENTLEIN left on the detached sperm head. These results demonstrate that CENTLEIN cooperating with SUN5 and PMFBP1 participates in the HTCA assembly and integration of sperm head to the tail, indicating that impairments of CENTLEIN might be associated with acephalic spermatozoa syndrome in humans. Nature Publishing Group UK 2021-08-13 /pmc/articles/PMC8363609/ /pubmed/34389728 http://dx.doi.org/10.1038/s41467-021-25227-w Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Zhang, Ying Liu, Chao Wu, Bingbing Li, Liansheng Li, Wei Yuan, Li The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus |
title | The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus |
title_full | The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus |
title_fullStr | The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus |
title_full_unstemmed | The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus |
title_short | The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus |
title_sort | missing linker between sun5 and pmfbp1 in sperm head-tail coupling apparatus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363609/ https://www.ncbi.nlm.nih.gov/pubmed/34389728 http://dx.doi.org/10.1038/s41467-021-25227-w |
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