New insights into the construction of wild-type Saba pig-derived Escherichia coli irp2 gene deletion strains

To construct wild-type E. coli irp2 gene deletion strains, CRISPR/Cas9 gene editing technology was used, and the difficulty and key points of gene editing of wild-type strains were analyzed. Based on the resistance of the CRISPR/Cas9 system expression vector, 4 strains of 41 E. coli strains isolated...

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Detalles Bibliográficos
Autores principales: Zhang, Bo, Wang, Hongdan, Zhao, Weiwei, Shan, Chunlan, Liu, Chaoying, Gao, Libo, Zhao, Ru, Ao, Pingxing, Xiao, Peng, Lv, Longbao, Gao, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363713/
https://www.ncbi.nlm.nih.gov/pubmed/34466347
http://dx.doi.org/10.1007/s13205-021-02951-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363713/
https://www.ncbi.nlm.nih.gov/pubmed/34466347
http://dx.doi.org/10.1007/s13205-021-02951-0

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