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In vitro turnover numbers do not reflect in vivo activities of yeast enzymes

Turnover numbers (k(cat) values) quantitatively represent the activity of enzymes, which are mostly measured in vitro. While a few studies have reported in vivo catalytic rates (k(app) values) in bacteria, a large-scale estimation of k(app) in eukaryotes is lacking. Here, we estimated k(app) of the...

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Detalles Bibliográficos
Autores principales: Chen, Yu, Nielsen, Jens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364156/
https://www.ncbi.nlm.nih.gov/pubmed/34341111
http://dx.doi.org/10.1073/pnas.2108391118