Cargando…
In vitro turnover numbers do not reflect in vivo activities of yeast enzymes
Turnover numbers (k(cat) values) quantitatively represent the activity of enzymes, which are mostly measured in vitro. While a few studies have reported in vivo catalytic rates (k(app) values) in bacteria, a large-scale estimation of k(app) in eukaryotes is lacking. Here, we estimated k(app) of the...
Autores principales: | Chen, Yu, Nielsen, Jens |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8364156/ https://www.ncbi.nlm.nih.gov/pubmed/34341111 http://dx.doi.org/10.1073/pnas.2108391118 |
Ejemplares similares
-
Kinetic profiling of metabolic specialists demonstrates stability and consistency of in vivo enzyme turnover numbers
por: Heckmann, David, et al.
Publicado: (2020) -
Redirection of lipid flux toward phospholipids in yeast increases fatty acid turnover and secretion
por: Ferreira, Raphael, et al.
Publicado: (2018) -
Yeast optimizes metal utilization based on metabolic network and enzyme kinetics
por: Chen, Yu, et al.
Publicado: (2021) -
Yeast has evolved to minimize protein resource cost for synthesizing amino acids
por: Chen, Yu, et al.
Publicado: (2022) -
Adaptations in metabolism and protein translation give rise to the Crabtree effect in yeast
por: Malina, Carl, et al.
Publicado: (2021)