A genotype-phenotype screening system using conditionally immortalized immature dendritic cells

Here, we describe a protocol for CRISPR/Cas9-mediated gene knockout in conditionally immortalized immature dendritic cells (DCs), which can be limitlessly expanded before differentiation. This facilitates the genetic screening of DC functions in vitro including assessment of phagocytosis, cytokine p...

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Detalles Bibliográficos
Autores principales: Zhao, Liwei, Liu, Peng, Xie, Wei, Zhang, Shuai, Thieme, Sebastian, Zitvogel, Laurence, Kroemer, Guido, Kepp, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365513/
https://www.ncbi.nlm.nih.gov/pubmed/34430908
http://dx.doi.org/10.1016/j.xpro.2021.100732

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8365513/
https://www.ncbi.nlm.nih.gov/pubmed/34430908
http://dx.doi.org/10.1016/j.xpro.2021.100732

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