High-salt transcription of DNA cotethered with T7 RNA polymerase to beads generates increased yields of highly pure RNA

High yields of RNA are routinely prepared following the two-step approach of high-yield in vitro transcription using T7 RNA polymerase followed by extensive purification using gel separation or chromatographic methods. We recently demonstrated that in high-yield transcription reactions, as RNA accum...

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Detalles Bibliográficos
Autores principales: Cavac, Elvan, Ramírez-Tapia, Luis E., Martin, Craig T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368030/
https://www.ncbi.nlm.nih.gov/pubmed/34303704
http://dx.doi.org/10.1016/j.jbc.2021.100999

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368030/
https://www.ncbi.nlm.nih.gov/pubmed/34303704
http://dx.doi.org/10.1016/j.jbc.2021.100999

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