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High-salt transcription of DNA cotethered with T7 RNA polymerase to beads generates increased yields of highly pure RNA
High yields of RNA are routinely prepared following the two-step approach of high-yield in vitro transcription using T7 RNA polymerase followed by extensive purification using gel separation or chromatographic methods. We recently demonstrated that in high-yield transcription reactions, as RNA accum...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society for Biochemistry and Molecular Biology
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368030/ https://www.ncbi.nlm.nih.gov/pubmed/34303704 http://dx.doi.org/10.1016/j.jbc.2021.100999 |