Reduced off-target effect of NG-BE4max by using NG-HiFi system

Recently, a rationally engineered SpCas9 variant (SpCas9-NG) that can recognize a minimal NG protospacer adjacent motif (PAM) was reported to expand the targeting scope in genome editing. However, increased genome-wide off-target mutations with this variant compared with SpCas9 were reported in prev...

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Detalles Bibliográficos
Autores principales: Shan, Huanhuan, Liu, Zhiquan, Jia, Yingqi, Chen, Siyu, Chen, Mao, Song, Yuning, Sui, Tingting, Lai, Liangxue, Li, Zhanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368781/
https://www.ncbi.nlm.nih.gov/pubmed/34458002
http://dx.doi.org/10.1016/j.omtn.2021.05.012

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8368781/
https://www.ncbi.nlm.nih.gov/pubmed/34458002
http://dx.doi.org/10.1016/j.omtn.2021.05.012

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