Fluorescent tagging of endogenous proteins with CRISPR/Cas9 in primary mouse neural stem cells

Although exogenous overexpression of a protein fused to a fluorescent tag can provide insight for the protein’s function, it also can produce artifacts attributed to its upregulation and may not fully report the endogenous regulation of the protein of interest. To circumvent these issues, we adapted...

Täydet tiedot

Bibliografiset tiedot
Päätekijät: Morrow, Christopher S., Porter, Tiaira J., Moore, Darcie L.
Aineistotyyppi: Online Artikkeli Teksti
Kieli:English
Julkaistu: Elsevier 2021
Aiheet:
Protocol
Linkit:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369070/
https://www.ncbi.nlm.nih.gov/pubmed/34430917
http://dx.doi.org/10.1016/j.xpro.2021.100744

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369070/
https://www.ncbi.nlm.nih.gov/pubmed/34430917
http://dx.doi.org/10.1016/j.xpro.2021.100744

Samankaltaisia teoksia