Fluorescent tagging of endogenous proteins with CRISPR/Cas9 in primary mouse neural stem cells

Although exogenous overexpression of a protein fused to a fluorescent tag can provide insight for the protein’s function, it also can produce artifacts attributed to its upregulation and may not fully report the endogenous regulation of the protein of interest. To circumvent these issues, we adapted...

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Detalles Bibliográficos
Autores principales: Morrow, Christopher S., Porter, Tiaira J., Moore, Darcie L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369070/
https://www.ncbi.nlm.nih.gov/pubmed/34430917
http://dx.doi.org/10.1016/j.xpro.2021.100744

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369070/
https://www.ncbi.nlm.nih.gov/pubmed/34430917
http://dx.doi.org/10.1016/j.xpro.2021.100744

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