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Fluorescent tagging of endogenous proteins with CRISPR/Cas9 in primary mouse neural stem cells
Although exogenous overexpression of a protein fused to a fluorescent tag can provide insight for the protein’s function, it also can produce artifacts attributed to its upregulation and may not fully report the endogenous regulation of the protein of interest. To circumvent these issues, we adapted...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369070/ https://www.ncbi.nlm.nih.gov/pubmed/34430917 http://dx.doi.org/10.1016/j.xpro.2021.100744 |