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RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis
CLP1, TSEN complex, and VCP are evolutionarily conserved proteins whose mutations are associated with neurodegenerative diseases. In this study, we have found that they are also involved in germline differentiation. To optimize both quantity and quality in gametes production, germ cells expand thems...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369837/ https://www.ncbi.nlm.nih.gov/pubmed/33864361 http://dx.doi.org/10.1093/hmg/ddab107 |
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author | Wu, Jianbo Li, Xin Gao, Zhiyang Pang, Lin Liu, Xian Huang, Xiahe Wang, Yingchun Wang, Zhaohui |
author_facet | Wu, Jianbo Li, Xin Gao, Zhiyang Pang, Lin Liu, Xian Huang, Xiahe Wang, Yingchun Wang, Zhaohui |
author_sort | Wu, Jianbo |
collection | PubMed |
description | CLP1, TSEN complex, and VCP are evolutionarily conserved proteins whose mutations are associated with neurodegenerative diseases. In this study, we have found that they are also involved in germline differentiation. To optimize both quantity and quality in gametes production, germ cells expand themselves through limited mitotic cycles prior to meiosis. Stemming from our previous findings on the correlation between mRNA 3′-processing and meiosis entry, here we identify that the RNA kinase Cbc, the Drosophila member of the highly conserved CLP1 family, is a component of the program regulating the transition from mitosis to meiosis. Using genetic manipulations in Drosophila testis, we demonstrate that nuclear Cbc is required to promote meiosis entry. Combining biochemical and genetic methods, we reveal that Cbc physically and/or genetically intersects with Tsen54 and TER94 (VCP ortholog) in this process. The C-terminal half of Tsen54 is both necessary and sufficient for its binding with Cbc. Further, we illustrate the functional conservation between Cbc and mammalian CLP1 in the assays of subcellular localization and Drosophila fertility. As CLP1, TSEN complex, and VCP have also been identified in neurodegenerations of animal models, a mechanism involving these factors seems to be shared in gametogenesis and neurogenesis. |
format | Online Article Text |
id | pubmed-8369837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-83698372021-08-18 RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis Wu, Jianbo Li, Xin Gao, Zhiyang Pang, Lin Liu, Xian Huang, Xiahe Wang, Yingchun Wang, Zhaohui Hum Mol Genet General Article CLP1, TSEN complex, and VCP are evolutionarily conserved proteins whose mutations are associated with neurodegenerative diseases. In this study, we have found that they are also involved in germline differentiation. To optimize both quantity and quality in gametes production, germ cells expand themselves through limited mitotic cycles prior to meiosis. Stemming from our previous findings on the correlation between mRNA 3′-processing and meiosis entry, here we identify that the RNA kinase Cbc, the Drosophila member of the highly conserved CLP1 family, is a component of the program regulating the transition from mitosis to meiosis. Using genetic manipulations in Drosophila testis, we demonstrate that nuclear Cbc is required to promote meiosis entry. Combining biochemical and genetic methods, we reveal that Cbc physically and/or genetically intersects with Tsen54 and TER94 (VCP ortholog) in this process. The C-terminal half of Tsen54 is both necessary and sufficient for its binding with Cbc. Further, we illustrate the functional conservation between Cbc and mammalian CLP1 in the assays of subcellular localization and Drosophila fertility. As CLP1, TSEN complex, and VCP have also been identified in neurodegenerations of animal models, a mechanism involving these factors seems to be shared in gametogenesis and neurogenesis. Oxford University Press 2021-04-16 /pmc/articles/PMC8369837/ /pubmed/33864361 http://dx.doi.org/10.1093/hmg/ddab107 Text en © The Author(s) 2021. Published by Oxford University Press. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | General Article Wu, Jianbo Li, Xin Gao, Zhiyang Pang, Lin Liu, Xian Huang, Xiahe Wang, Yingchun Wang, Zhaohui RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis |
title | RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis |
title_full | RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis |
title_fullStr | RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis |
title_full_unstemmed | RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis |
title_short | RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis |
title_sort | rna kinase clp1/cbc regulates meiosis initiation in spermatogenesis |
topic | General Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8369837/ https://www.ncbi.nlm.nih.gov/pubmed/33864361 http://dx.doi.org/10.1093/hmg/ddab107 |
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