Isolation of autophagic fractions from mouse liver for biochemical analyses

Isolation of autophagosomes, autolysosomes, and lysosomes allows mechanistic studies into the pathophysiology of autophagy—a lysosomal quality control pathway. Here, we outline a Nycodenz density gradient ultracentrifugation approach for high-yield isolation of autophagic fractions from mouse liver....

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Detalles Bibliográficos
Autores principales: Bains, Henrietta, Singh, Rajat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371247/
https://www.ncbi.nlm.nih.gov/pubmed/34430907
http://dx.doi.org/10.1016/j.xpro.2021.100730

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8371247/
https://www.ncbi.nlm.nih.gov/pubmed/34430907
http://dx.doi.org/10.1016/j.xpro.2021.100730

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