Plasmid replication based on the T7 origin of replication requires a T7 RNAP variant and inactivation of ribonuclease H

T7 RNA polymerase (RNAP) is a valuable tool in biotechnology, basic research and synthetic biology due to its robust, efficient and selective transcription of genes. Here, we expand the scope of T7 RNAP to include plasmid replication. We present a novel type of plasmid, termed T7 ori plasmids that r...

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Detalles Bibliográficos
Autores principales: Becker, Katja, Meyer, Andreas, Roberts, Tania Michelle, Panke, Sven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Genome Integrity, Repair and Replication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373140/
https://www.ncbi.nlm.nih.gov/pubmed/34255845
http://dx.doi.org/10.1093/nar/gkab596

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373140/
https://www.ncbi.nlm.nih.gov/pubmed/34255845
http://dx.doi.org/10.1093/nar/gkab596

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