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Plasmid replication based on the T7 origin of replication requires a T7 RNAP variant and inactivation of ribonuclease H
T7 RNA polymerase (RNAP) is a valuable tool in biotechnology, basic research and synthetic biology due to its robust, efficient and selective transcription of genes. Here, we expand the scope of T7 RNAP to include plasmid replication. We present a novel type of plasmid, termed T7 ori plasmids that r...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8373140/ https://www.ncbi.nlm.nih.gov/pubmed/34255845 http://dx.doi.org/10.1093/nar/gkab596 |